Résumé du séminaire : 

Due to its asymmetrical division pattern and finite lifespan, budding yeast provide an interesting model to understand the fundamental mechanisms that limit replicative longevity. In the last decades, several independent models have been proposed to explain the mechanism of entry into replicative senescence in yeast, thus questioning whether and how aging is driven by multifactorial causes. Yet, classical lifespan assays make it very difficult to disentangle the choreography of events leading to senescence. In the last ten years, we and others pioneered the development of microfluidics-based imaging methods to track the successive division of individual cells from birth to death under the microscope. Such a dynamic analysis has started to unravel the mechanism that drives the entry into replicative senescence.